標(biāo)題: Titlebook: African Swine Fever Virus; Methods and Protocol Christopher L. Netherton Book 2022 The Editor(s) (if applicable) and The Author(s), under e [打印本頁] 作者: 要求 時(shí)間: 2025-3-21 17:22
書目名稱African Swine Fever Virus影響因子(影響力)
作者: 昆蟲 時(shí)間: 2025-3-21 22:22
Necropsy Procedures and Evaluation of Macroscopic Lesions of Pigs Infected with African Swine Fevergy, or virology as core basis of scientific research. Necropsies are an essential tool in veterinary pathology for disease investigation and should be conducted in a routine, systematic, and standard approach. An orderly necropsy procedure will allow the prosector (veterinary clinicians or veterinar作者: 成績上升 時(shí)間: 2025-3-22 02:35 作者: 充滿裝飾 時(shí)間: 2025-3-22 04:43 作者: Alveolar-Bone 時(shí)間: 2025-3-22 12:33
Isolation of Porcine Bone Marrow Cells and Generation of Recombinant African Swine Fever Viruses,further our understanding of the virus–host interactions. Here we present methods for isolation of porcine bone marrow cells and purification of recombinant ASFV using both chromogenic and fluorescent reporters. We also describe in detail a newly developed method to purify genetically modified ASFV 作者: sparse 時(shí)間: 2025-3-22 15:01 作者: Neutropenia 時(shí)間: 2025-3-22 19:35 作者: 群居動物 時(shí)間: 2025-3-23 00:11
Genotyping of African Swine Fever Virus,is known as the gold standard diagnostic method for most diseases and is also used for the detection of African swine fever virus (ASFV) DNA in clinical specimens. To determine the ASFV genotype or identify additional genome markers, endpoint PCR is usually performed on ASFV-positive specimens, foll作者: 漸強(qiáng) 時(shí)間: 2025-3-23 02:41 作者: paleolithic 時(shí)間: 2025-3-23 07:45
Indirect Immunoperoxidase Test (IPT) for Detection of Antibodies Against African Swine Fever Virus hrough the action of the peroxidase enzyme. In this procedure, African green monkey established cell lines, such as Vero or MS, are infected with African swine fever virus (ASFV)-adapted isolates to these cell cultures. The infected cells are fixed and then used as antigens to determine the presence作者: 要求比…更好 時(shí)間: 2025-3-23 10:32
African Swine Fever Virus Hemadsorption Inhibition Assay,wing to determine virus infectious titer in hemadsorption unit (HAU) and differentiate virus strains phenotypically. In the meantime, hemadsorption of particular ASFV strain can by inhibited by homologous anti-ASFV serum containing antibody to the serogroup-specific virus protein (CD2v). Here, we de作者: 脖子 時(shí)間: 2025-3-23 16:42 作者: Fulminate 時(shí)間: 2025-3-23 20:01 作者: 確定無疑 時(shí)間: 2025-3-24 01:35 作者: 說不出 時(shí)間: 2025-3-24 05:44 作者: epicardium 時(shí)間: 2025-3-24 07:23
Whole Genome Sequencing of African Swine Fever, infectious disease. It enables high-resolution characterization of viral pathogens in terms of properties that include molecular epidemiology, genotype, serotype, and virulence. However, a beginner’s NGS protocol for characterization of African swine fever virus (ASFV) is lacking. Here, we present 作者: 放氣 時(shí)間: 2025-3-24 11:42
Isolation of Porcine Bone Marrow Cells and Generation of Recombinant African Swine Fever Viruses,further our understanding of the virus–host interactions. Here we present methods for isolation of porcine bone marrow cells and purification of recombinant ASFV using both chromogenic and fluorescent reporters. We also describe in detail a newly developed method to purify genetically modified ASFV using fluorescence-activated cell sorting (FACS).作者: aspect 時(shí)間: 2025-3-24 18:11
Quantification of ASFV DNA and RNA in , Soft Ticks,se transcription followed by a polymerase chain reaction (RT-PCR) permit the detection of the presence and the replication of African swine fever virus in soft ticks. Here, we described our techniques to detect and quantify DNA and RNA of African swine fever virus in soft ticks including a housekeeping gene of soft ticks as internal control.作者: 憎惡 時(shí)間: 2025-3-24 22:33 作者: minion 時(shí)間: 2025-3-24 23:48 作者: 平常 時(shí)間: 2025-3-25 04:55
https://doi.org/10.1057/9780312299590se transcription followed by a polymerase chain reaction (RT-PCR) permit the detection of the presence and the replication of African swine fever virus in soft ticks. Here, we described our techniques to detect and quantify DNA and RNA of African swine fever virus in soft ticks including a housekeeping gene of soft ticks as internal control.作者: ADORE 時(shí)間: 2025-3-25 07:40
https://doi.org/10.1007/978-1-0716-2333-6Immunology; Vaccinology; Pig disease; Mammalian hosts; Arthropod hosts作者: 雀斑 時(shí)間: 2025-3-25 14:42
978-1-0716-2335-0The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: Debrief 時(shí)間: 2025-3-25 18:27 作者: 膽大 時(shí)間: 2025-3-25 23:09
https://doi.org/10.1007/978-1-4842-6291-7gy, or virology as core basis of scientific research. Necropsies are an essential tool in veterinary pathology for disease investigation and should be conducted in a routine, systematic, and standard approach. An orderly necropsy procedure will allow the prosector (veterinary clinicians or veterinar作者: Migratory 時(shí)間: 2025-3-26 03:10 作者: 樸素 時(shí)間: 2025-3-26 04:20 作者: Antimicrobial 時(shí)間: 2025-3-26 10:10 作者: Binge-Drinking 時(shí)間: 2025-3-26 14:37 作者: Comprise 時(shí)間: 2025-3-26 17:57 作者: 附錄 時(shí)間: 2025-3-27 00:26
Creating a Dialectical Social Scienceis known as the gold standard diagnostic method for most diseases and is also used for the detection of African swine fever virus (ASFV) DNA in clinical specimens. To determine the ASFV genotype or identify additional genome markers, endpoint PCR is usually performed on ASFV-positive specimens, foll作者: Bombast 時(shí)間: 2025-3-27 04:01
Ian I. Mitroff,Richard O. Masonon the use of the soluble cytoplasmic fraction of ASFV-infected monkey stable cells (MS). The soluble antigen proteins of ASFV-infected cells are separated by sucrose precipitation centrifugation, and the supernatant above the sucrose layer is used as an ELISA antigen. The test serum sample reacts w作者: CLAMP 時(shí)間: 2025-3-27 05:48 作者: Grasping 時(shí)間: 2025-3-27 13:02 作者: Concerto 時(shí)間: 2025-3-27 16:21 作者: adduction 時(shí)間: 2025-3-27 18:04
https://doi.org/10.1007/978-981-16-3276-1f virus-infected cells are routinely used for virological and immunological studies, despite differences in the biological behavior between such preparations and highly purified virus. In addition, more recent data suggests that exosomes containing viral proteins may be secreted from infected cells.作者: Keratin 時(shí)間: 2025-3-28 00:12
Robert F. Kushner,Jeffrey I. Mechanickes such as real-time PCR and virus isolation provide an understanding as to whether viral genome is present or gives a qualitative assessment of live viral presence in a sample respectively, but neither provide a quantitative measurement of live virus. Here we describe a plaque assay for the titrati作者: ORE 時(shí)間: 2025-3-28 05:53 作者: 擁擠前 時(shí)間: 2025-3-28 09:16 作者: Infirm 時(shí)間: 2025-3-28 12:02 作者: 燦爛 時(shí)間: 2025-3-28 18:08
Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/150846.jpg作者: Guileless 時(shí)間: 2025-3-28 19:21 作者: Headstrong 時(shí)間: 2025-3-28 23:14
African Swine Fever Virus (ASFV) Indirect ELISA Test Based on the Use of the Soluble Cytoplasmic Seith the cytoplasmic soluble fraction, and antibodies are detected using a protein A-peroxidase conjugate. This crude antigen is currently recommended as a test reagent in screening and diagnostic tests by the World Organization for Animal Health (OIE).作者: 直言不諱 時(shí)間: 2025-3-29 04:53 作者: 喪失 時(shí)間: 2025-3-29 10:00 作者: 說明 時(shí)間: 2025-3-29 13:03 作者: 不透明性 時(shí)間: 2025-3-29 17:21 作者: florid 時(shí)間: 2025-3-29 23:39
https://doi.org/10.1007/978-981-16-3276-1rations and highly purified virus. In addition, more recent data suggests that exosomes containing viral proteins may be secreted from infected cells. While African swine fever virus can be purified through a number of methods, in our hands Percoll provides the most robust method of separating virus from cellular contaminants.作者: MULTI 時(shí)間: 2025-3-30 00:28
Robert F. Kushner,Jeffrey I. Mechanickviral presence in a sample respectively, but neither provide a quantitative measurement of live virus. Here we describe a plaque assay for the titration of a Vero-adapted African swine fever virus strain (BA71V) and describe how to apply this method to determine disinfectant efficacy.作者: atopic 時(shí)間: 2025-3-30 05:28 作者: 減震 時(shí)間: 2025-3-30 10:03 作者: MOAN 時(shí)間: 2025-3-30 15:23 作者: 相一致 時(shí)間: 2025-3-30 18:58 作者: CAB 時(shí)間: 2025-3-31 00:40 作者: Lineage 時(shí)間: 2025-3-31 04:46
Yulia Vymyatnina,Daria Antonovahe HADIA is a powerful method in the ASFV immunopathology and vaccine research since it provides additional antigenic and phenotypic characteristics of virus strains that can’t be defined by other assays.作者: Infuriate 時(shí)間: 2025-3-31 06:48
Preparation of Immunofluorescently Labeled Tissue Sections for Imaging at Low and High Magnificatioon of multiple proteins and structures. In this chapter, we describe the technique used to prepare vibrating microtome sections, using porcine tissue infected with African swine fever virus as an example. This technique can easily be applied to any animal tissue with any suitable combination of antibodies, depending on the hypothesis.作者: sleep-spindles 時(shí)間: 2025-3-31 11:07
African Swine Fever Virus Hemadsorption Inhibition Assay,he HADIA is a powerful method in the ASFV immunopathology and vaccine research since it provides additional antigenic and phenotypic characteristics of virus strains that can’t be defined by other assays.作者: 輕快走過 時(shí)間: 2025-3-31 16:17 作者: 惡心 時(shí)間: 2025-3-31 18:24
Creating a Lifestyle Medicine Centerresents a step-by-step and reproducible method for producing high-quality sequencing data. The key advantages of this protocol include the protocol being very simple for users with no experience of genome sequencing and reproducibility of the protocol for other DNA genome viruses.
