標(biāo)題: Titlebook: Activity-Based Proteomics; Methods and Protocol Herman S. Overkleeft,Bogdan I. Florea Book 2017 Springer Science+Business Media New York 20 [打印本頁(yè)] 作者: minuscule 時(shí)間: 2025-3-21 19:05
書(shū)目名稱Activity-Based Proteomics影響因子(影響力)
作者: athlete’s-foot 時(shí)間: 2025-3-21 23:03 作者: 從容 時(shí)間: 2025-3-22 01:25 作者: 我說(shuō)不重要 時(shí)間: 2025-3-22 04:35 作者: fatty-streak 時(shí)間: 2025-3-22 09:10 作者: 星球的光亮度 時(shí)間: 2025-3-22 14:14 作者: Melanoma 時(shí)間: 2025-3-22 20:58 作者: GLUE 時(shí)間: 2025-3-23 00:32
Activity-Based Lipid Esterase Profiling of , BCG at Different Metabolic States Using Tetrahydrolipst later, Florsheim and Morton (1957) showed that phospholipid exchange between serum lipoprotein fractions is rapid compared to the disappearance of these lipids from plasma. Exchange of phospholipids between chylomicrons and higher density serum lipoprotein was demonstrated by McCandless and Zilvers作者: anaerobic 時(shí)間: 2025-3-23 03:03 作者: 懶惰民族 時(shí)間: 2025-3-23 08:00
cts which are required by the organism and are otherwise unavailable. As such, the overall metabolic conversion of substrate to product must be associated with a negative free energy change and will not be at equilibrium. Analysis of the reactants in various enzymic reactions has established, howeve作者: pester 時(shí)間: 2025-3-23 12:31 作者: critic 時(shí)間: 2025-3-23 16:19
Profiling the Activity of Deubiquitinating Enzymes Using Chemically Synthesized Ubiquitin-Based Probent biophysics.Only book which covers all of the 10 currentl.Incorporating recent dramatic advances, this textbook presents a fresh and timely introduction to modern biophysical methods. An array of new, faster and higher-power biophysical methods now enables scientists to examine the mysteries of l作者: 殘忍 時(shí)間: 2025-3-23 18:41
Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells for the global characterization of molecular conformations and conformational changes of proteins and other biomolecules. Depending on the measurement technique, scanning infrared (IR) spectrometers, Fourier transform infrared (FTIR) spectrometers, and single wavelength infrared apparatuses are dis作者: 不感興趣 時(shí)間: 2025-3-23 23:14
Live Cell Imaging and Profiling of Cysteine Cathepsin Activity Using a Quenched Activity-Based Probe and higher-power biophysical methods now enables scientists to examine the mysteries of life at a molecular level. This innovative text surveys and explains the ten key biophysical methods, including those related to biophysical nanotechnology, scanning probe microscopy, X-ray crystallography, ion 作者: Antimicrobial 時(shí)間: 2025-3-24 05:31 作者: Pulmonary-Veins 時(shí)間: 2025-3-24 09:42
eks to understand the neural basis of natural animal behavior. Novel approaches including molecular biological techniques, optical recording methods, functional anatomy, and informatics have brought drastic changes in how the neural systems underlying high-level behaviors such as learning and memory作者: aquatic 時(shí)間: 2025-3-24 13:18
A Protocol for Protein Profiling Using Chemoselective Cleavable Linker Probes in Semi-permeabilized stive. Common natural substrates are triglycerides and derivatives thereof such as di-and monoglycerides. In contrast to carboxyl esterases (EC 3.1.1.1), they act efficiently on water-insoluble substrates by binding to the water-organic interface, whereas the organic interface usually consists of a 作者: Fissure 時(shí)間: 2025-3-24 15:03
Two-Step Activity-Based Protein Profiling with the Proteasome System as Model of Studyf ionic channels (Strichartz, 1973; Courtney, 1975; Khodorov .., 1976; Hille, 1977; Hondeghem and Katzung, 1977). In the 1980s this concept was applied to the mechanism of action of calcium channel ligands on L-type calcium channels in heart (Bean, 1984; Sanguinetti and Kass, 1984; McDonald .., 1984作者: PATRI 時(shí)間: 2025-3-24 21:41
Back Matterough all of these genes are not expressed at any particular time, any individual cell may synthesize several thousand different proteins. Determining the intracellular localization of proteins is an essential part of elucidating function. Localization can present further complications because protei作者: Inscrutable 時(shí)間: 2025-3-25 02:26
Alexander Haensch,Nicolae Barsan,Udo Weimar of enzyme activities by dissociation from inhibitors, activation/deactivation through posttranslational modification, or relocation of the enzyme to different organelles. This means that expression levels of enzymes do not necessarily correlate with the activities observed for these enzymes. In thi作者: Harridan 時(shí)間: 2025-3-25 06:43 作者: conformity 時(shí)間: 2025-3-25 08:22 作者: 售穴 時(shí)間: 2025-3-25 13:57 作者: Infelicity 時(shí)間: 2025-3-25 19:44
Desigm Mission für die Automobilindustrieespecially in view of an alarming increase in bacterial resistance to existing antibiotics and antibacterial agents. Use of small molecules to dissect the basis of these dynamic processes is a very attractive approach due to their ability for rapid spatiotemporal control of specific biochemical func作者: 外面 時(shí)間: 2025-3-25 20:27 作者: –scent 時(shí)間: 2025-3-26 03:39
Desigm Mission für die Automobilindustrieions in their native cellular environment. One of the biggest challenges for ABPP is the extension of the proteome coverage. In this chapter a new ABPP strategy dedicated to monoamine oxidases (MAO) is presented. These enzymes are representative examples of flavin-dependent oxidases, playing a cruci作者: 注意 時(shí)間: 2025-3-26 08:04
Elemente der Automobilbranche im Umbruch, the accurate profiling of DUB activity as well as the identification of DUBs and DUB inhibitor selectivity. Previously, active-site directed DUB probes have been obtained using intein-based methods that have inherent limitations. Total chemical synthesis of ubiquitin allows for easy incorporation o作者: 詞根詞綴法 時(shí)間: 2025-3-26 09:05 作者: Nostalgia 時(shí)間: 2025-3-26 13:46
https://doi.org/10.1007/978-3-531-91576-0of enzyme inhibitors in complex proteomes. Fluorophosphonate-based fluorescent inhibitors are widely used as broad-spectrum probes for serine hydrolases. However, diacylglycerol lipase-α is not labeled by fluorophosphonate-based probes. To overcome this problem, we have developed a tailor-made activ作者: arthrodesis 時(shí)間: 2025-3-26 18:14 作者: circumvent 時(shí)間: 2025-3-26 23:07
https://doi.org/10.1007/978-3-531-91576-0rometry is the primary method for target identification, and in the last decade, cleavable linkers have become a popular strategy to facilitate protein enrichment and identification. In this chapter, we provide an overview of cleavable linkers used in chemical proteomics approaches, discuss their di作者: 集聚成團(tuán) 時(shí)間: 2025-3-27 02:08 作者: 航海太平洋 時(shí)間: 2025-3-27 06:51
https://doi.org/10.1007/978-1-4939-6439-0activity-based proteomics; annotate enzyme families; activity-based protein; bioorthogonal chemistry; an作者: 無(wú)所不知 時(shí)間: 2025-3-27 12:38
978-1-4939-8200-4Springer Science+Business Media New York 2017作者: 軍械庫(kù) 時(shí)間: 2025-3-27 15:32
Activity-Based Proteomics978-1-4939-6439-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: MAIZE 時(shí)間: 2025-3-27 19:03
Nils Labahn,Fabian Uterm?hlen,Harry Weberocol, we describe the workflow for an activity-based protein profiling (ABPP) experiment with an alkyne-tagged natural product derivative. Our protocol includes experimental procedures for in vivo labeling, sample preparation and 2-step (click chemistry) visualization and sample preparation for mass spectrometry-based target identification.作者: Dungeon 時(shí)間: 2025-3-28 01:44 作者: 主動(dòng) 時(shí)間: 2025-3-28 02:36 作者: filicide 時(shí)間: 2025-3-28 08:39
Book 2017vity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily repro作者: 輕推 時(shí)間: 2025-3-28 12:33 作者: Middle-Ear 時(shí)間: 2025-3-28 16:35 作者: 射手座 時(shí)間: 2025-3-28 22:10 作者: 卵石 時(shí)間: 2025-3-29 01:34
Elemente der Automobilbranche im Umbruch,, an in-gel activity-based DUB profiling assay is described in detail, in which the fluorescent DUB probe is used to visualize active DUBs in cell lysates. Finally, an inhibition assay is described in which the fluorescent probe is used to determine the specificity and potency of a small molecule DUB inhibitor.作者: MOCK 時(shí)間: 2025-3-29 04:05
https://doi.org/10.1007/978-3-531-91576-0owed by an alkyne-substituted pan-reactive probe to label the remaining β2 activity to which an azide-coupled fluorophore was ligated. The result of the triple ligation was similar to each reaction performed separately demonstrating the value of the triple ligation strategy for a single experiment.作者: thwart 時(shí)間: 2025-3-29 08:36 作者: 他去就結(jié)束 時(shí)間: 2025-3-29 14:08 作者: Proclaim 時(shí)間: 2025-3-29 15:55 作者: 造反,叛亂 時(shí)間: 2025-3-29 22:26 作者: 構(gòu)想 時(shí)間: 2025-3-30 01:29 作者: 試驗(yàn) 時(shí)間: 2025-3-30 07:58
Desigm Mission für die Automobilindustriea powerful tool to study bacterial pathogenesis. In this chapter, we present a set of ABPP methods to identify and analyze enzymes essential for growth, metabolism and virulence of different pathogens including . and . using natural product-inspired activity-based probes.作者: Ligament 時(shí)間: 2025-3-30 11:29
Automobiles Design und Modernismusl activities, but its precise spectrum of molecular targets is poorly characterized. Here, we used THL analogs functionalized to enable Huisgen-base cycloaddition, commonly known as “click chemistry,” to identify target proteins after enrichment from mycobacterial cell lysates obtained from different physiological conditions.作者: Mindfulness 時(shí)間: 2025-3-30 13:38 作者: 一大塊 時(shí)間: 2025-3-30 19:23 作者: 闡釋 時(shí)間: 2025-3-30 22:44 作者: 哥哥噴涌而出 時(shí)間: 2025-3-31 01:33
Front Matterissue-culture cells for bio- chemical analysis; it thus meets the ever-increasing requirements of a modern cell-culture laboratory. To paraphrase the aims of this book, we quote part of the preface to the German edition: "It was intended to collect the various methods so as to make them accessible for laborat978-3-642-65789-4978-3-642-65787-0作者: 一罵死割除 時(shí)間: 2025-3-31 08:31
