標題: Titlebook: Western Blotting; Methods and Protocol Biji T. Kurien,R. Hal Scofield Book 2015 Springer Science+Business Media New York 2015 Antigenic Pep [打印本頁] 作者: FERAL 時間: 2025-3-21 17:05
書目名稱Western Blotting影響因子(影響力)
書目名稱Western Blotting影響因子(影響力)學(xué)科排名
書目名稱Western Blotting網(wǎng)絡(luò)公開度
書目名稱Western Blotting網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Western Blotting被引頻次
書目名稱Western Blotting被引頻次學(xué)科排名
書目名稱Western Blotting年度引用
書目名稱Western Blotting年度引用學(xué)科排名
書目名稱Western Blotting讀者反饋
書目名稱Western Blotting讀者反饋學(xué)科排名
作者: AWE 時間: 2025-3-21 21:06
Graeme Lowe Ph.D...Based on these findings, and taking into account of the ways and means against similar threat, such as terrorist attack and multiple operational errors, I would like to stress the importance of distributed arrangement and diversity of the safety components and systems.作者: 討人喜歡 時間: 2025-3-22 02:52
Joon-Yong Chung Ph.D.,Stephen M. Hewitt M.D., Ph.D., but from a technological perspective, in relation to the different component features. Related work relative to the component feature set is discussed. And, the component feature set is verified against the definitions of pervasive games.作者: ZEST 時間: 2025-3-22 07:28 作者: 推延 時間: 2025-3-22 10:24
Dot-Immunobinding Assay (Dot-Iba),tivity of 60 % for tuberculous meningitis diagnosis and no false positive results were encountered. Hence this assay is highly specific for the diagnosis of paucibacillary diseases like extrapulmonary tuberculosis. Dot-Iba is best suited to laboratories in developing world where there are constraint作者: averse 時間: 2025-3-22 15:14
Glycosaminoglycan Blotting and Detection After Electrophoresis Separation,onic detergent cetylpyridinium chloride (CPC) and mixtures of GAGs are capillary blotted after their separation in agarose-gel electrophoresis. Single purified species of variously sulfated polysaccharides are transferred on derivatized membranes with an efficiency of 100 % and stained with alcian b作者: 平庸的人或物 時間: 2025-3-22 19:46
A Well-Based Reverse-Phase Protein Array of Formalin-Fixed Paraffin-Embedded Tissue,he lysates coated to the array plate, dried up and vacuum-sealed, remain stable up to 2 months at room temperature. This methodology is directly applicable to FFPE tissue and presents the direct opportunity of addressing hypothesis within clinical trials and well-annotated clinical tissue repositori作者: 鞠躬 時間: 2025-3-22 21:21 作者: 憤世嫉俗者 時間: 2025-3-23 01:47 作者: FIR 時間: 2025-3-23 09:18 作者: phlegm 時間: 2025-3-23 11:45 作者: 身體萌芽 時間: 2025-3-23 16:31 作者: 軟膏 時間: 2025-3-23 19:32 作者: 壓倒性勝利 時間: 2025-3-23 23:50
Der-Yen Lee,Geen-Dong ChangOn the basis of inheriting and developing the former system, the appeal rule of the Tang Dynasty played an important role in realizing the appeal of the subject and preventing the abuse of the criminal penalty.作者: Critical 時間: 2025-3-24 03:37
Biji T. Kurien Ph.D.,R. Hal Scofield M.D.In summary, this book mainly presents the following findings: (i) at empirical level, differences exist in the time–space distribution of farmers’ suicide in China; and (ii) at theoretical level, suicide is not determined by social structure or suicidal action alone, but is a product of interplay between the two.作者: infantile 時間: 2025-3-24 09:16 作者: cultivated 時間: 2025-3-24 11:51
Dalit Talmi-Frank D.V.M., Ph.D.,Charles L. Jaffe Ph.D.,Gad Baneth D.V.M., Ph.D.t of IS research is devoted to understanding the effect of the IT on communication and collaboration, including communication between individuals, teamwork, and the creation and development of larger virtual communities.作者: 泥瓦匠 時間: 2025-3-24 18:35
Origins of Protein Blotting, elements of common methods and simple lab equipment resulted in a procedure of general utility. The expansion of the idea of carrying out immunoassays on membranes as predecessors of microarrays is briefly touched upon.作者: eulogize 時間: 2025-3-24 22:56
Western Blotting: An Introduction, the transfer of protein patterns from gel to microporous membrane. Electrophoretic as well as non-electrophoretic transfer of proteins to membranes was first described in 1979. Protein blotting has evolved greatly since the inception of this protocol, allowing protein transfer to be accomplished in a variety of ways.作者: oxidant 時間: 2025-3-25 00:42
From Little Helpers to Automation,his brief review outlines a representative selection of methods and devices, many of which are commercial products. In particular, the steps taken towards partial and full automation of western blotting are addressed.作者: Emasculate 時間: 2025-3-25 04:14
Cationic Electrophoresis and Eastern Blotting, where this method produces suboptimal results. In these cases electrophoresis in the presence of positively charged detergents like cetyltrimethylammonium bromide (CTAB) may work considerably better. Methods for electrophoresis, staining, and blotting of such gels are presented.作者: 業(yè)余愛好者 時間: 2025-3-25 10:40 作者: maculated 時間: 2025-3-25 14:13
https://doi.org/10.1007/978-1-4939-2694-7Antigenic Peptides; Centrifuge Blotting; Diffusion Blotting; Electroblotting; Immunoprecipitation; Protei作者: neolith 時間: 2025-3-25 19:45 作者: 錢財 時間: 2025-3-25 22:59
Edwin Southernalyzed. Also, the nature of the different types of bonds and their corresponding topological properties of electronic charge density are systematically and quantitatively investigated by using the NBO analysis and the atoms in molecules theory (AIM).978-94-007-5753-0978-94-007-5754-7Series ISSN 2191-5407 Series E-ISSN 2191-5415 作者: semble 時間: 2025-3-26 03:14
Harry Towbin Ph.D.hromyl fluorosulphate was performed. The nature of the Cr–O and Cr?←?O bonds and the topological properties of the compound were investigated and analyzed by means of natural bond order (NBO) and . atoms in molecules theory (AIM), respectively.作者: compel 時間: 2025-3-26 05:06 作者: 刻苦讀書 時間: 2025-3-26 10:00 作者: 自傳 時間: 2025-3-26 13:47
The Early Days of Blotting, fragments to a membrane support through capillary action, is also known as Southern blotting. This procedure enables the detection of a specific DNA sequence by hybridization with probes. The term Southern blotting led to a “geographic” naming tradition, with RNA blotting bearing the name Northern 作者: 攤位 時間: 2025-3-26 18:56
Origins of Protein Blotting, elements of common methods and simple lab equipment resulted in a procedure of general utility. The expansion of the idea of carrying out immunoassays on membranes as predecessors of microarrays is briefly touched upon.作者: nephritis 時間: 2025-3-26 21:54 作者: headlong 時間: 2025-3-27 05:05
Simian Virus 40 and Protein Transfer,tant tools in modern molecular and cellular biology. Since its development in 1979, the improvement of the technique has been impressive, from new apparatus to streamline the electrophoresis step to different modalities of the transfer step or solid supports for the transfer. Perhaps most impressive作者: cravat 時間: 2025-3-27 09:17
Western Blotting: An Introduction, the transfer of protein patterns from gel to microporous membrane. Electrophoretic as well as non-electrophoretic transfer of proteins to membranes was first described in 1979. Protein blotting has evolved greatly since the inception of this protocol, allowing protein transfer to be accomplished in作者: expunge 時間: 2025-3-27 13:00
From Little Helpers to Automation,his brief review outlines a representative selection of methods and devices, many of which are commercial products. In particular, the steps taken towards partial and full automation of western blotting are addressed.作者: Gingivitis 時間: 2025-3-27 15:59
Spectrophotometric Methods to Determine Protein Concentration,ecisions on the usefulness of any isolation procedure depend on knowing the relative concentrations of a particular protein or enzyme in relation to the concentrations of all the proteins present. Protein concentration in solution is generally measured with spectrophotometry in the UV range or in th作者: 固執(zhí)點好 時間: 2025-3-27 18:02
Solubilization of Proteins: The Importance of Lysis Buffer Choice,on of the focal adhesion protein Kindlin-2 from choriocarcinoma cells using NP-40 and RIPA lysis buffer. Furthermore, we demonstrate the use of a more denaturing urea/thiourea lysis buffer for solubilization, by comparing its effectiveness for solubilization of small heat-shock proteins from smooth 作者: Indecisive 時間: 2025-3-27 22:48 作者: Agnosia 時間: 2025-3-28 05:43 作者: parallelism 時間: 2025-3-28 09:21 作者: 聯(lián)想記憶 時間: 2025-3-28 10:46
Localizing Proteins by Tissue Printing,bohydrates, and small molecules in a tissue-specific mode. Plant tissues can be used to produce prints revealing a remarkable amount of anatomical detail, even without staining, which might be used to record developmental changes over time. In this chapter we will focus on the protocols for the loca作者: diskitis 時間: 2025-3-28 16:04
Dot-Immunobinding Assay (Dot-Iba),ose membrane (NCM) discs. The diagnostic material to be checked can be incubated on this disc. Presence of antigen-antibody complex in NCM discs can be directly demonstrated with enzyme-conjugated antiglobulins and substrate. Development of a purple-pink colored, insoluble substrate product in the n作者: Cirrhosis 時間: 2025-3-28 21:04
Analysis of Antibody Clonotype by Affinity Immunoblotting,n under observation is described. Patient sera, collected over several years, were separated by flatbed isoelectric focusing (IEF) and analyzed by affinity immunoblotting utilizing Ro 60-coated nitrocellulose membrane. When the Ro 60-coated nitrocellulose was laid over the surface of the IEF gel, th作者: pellagra 時間: 2025-3-29 02:13
Glycosaminoglycan Blotting and Detection After Electrophoresis Separation,h. Their blotting on membranes after electrophoresis offers the advantage to perform further analysis on single separated species such as identification with antibodies and/or recovery of single band. A method for the blotting and immobilizing of several nonsulfated and sulfated complex GAGs on memb作者: 珠寶 時間: 2025-3-29 06:01 作者: 領(lǐng)先 時間: 2025-3-29 10:15 作者: mechanical 時間: 2025-3-29 13:43 作者: Ligneous 時間: 2025-3-29 18:29
Book 2015oteins, single cell blotting and automated blotting. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and t作者: 合法 時間: 2025-3-29 20:47
W. Neal Burnette Ph.D.nge causes a qualitative change in the nature of the problem. We must expect therefore that once we include the ‘large’ in a problem we are most likely introducing with it a number of difficulties. This certainly applies to data structures which are in some sense ‘large’.作者: anthesis 時間: 2025-3-30 00:42
Mandy Peach,Noelle Marsh,Ewa I. Miskiewicz,Daniel J. MacPheefter a period of stagnation, China began to accelerate its foreign aid in the 1990s; it established the Export-Import (EXIM) Bank of China in 1994 and introduced concessional loans in 1995. Since then, China has rapidly expanded its activities in the developing world under its strategy of . (going out).作者: BILK 時間: 2025-3-30 04:43 作者: Benign 時間: 2025-3-30 09:25
Sumi Surendran M.Sc.,Annamma Mathai Ph.D.,Vishnampet Venkataraman Radhakrishnan M.D., F.A.M.S.rts: initial RAT selection and vertical handover (VHO). The former is used to allocate new calls to a suitable RAT and the latter is about transferring an ongoing call from its current serving RAT to a more suitable RAT.作者: CAMEO 時間: 2025-3-30 13:16 作者: Hemiplegia 時間: 2025-3-30 17:03 作者: Endemic 時間: 2025-3-30 21:27 作者: 擴張 時間: 2025-3-31 02:16
1064-3745 ation advice from the experts.This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful .Methods in Molecular Biology. seri作者: Repetitions 時間: 2025-3-31 05:36 作者: 減弱不好 時間: 2025-3-31 10:15
Nicola Volpi,Francesca Maccarir high energy physics. Second, we consider the case of an overdense plasma, where electrons are accelerated at the interface where the laser impinges. Such problem is strongly connected to the general issue of collisionless absorption in an overdense plasma, possibly the most complex and less understood topic of laser-plasma interactions. 作者: badinage 時間: 2025-3-31 14:51
The Early Days of Blotting,sequence by hybridization with probes. The term Southern blotting led to a “geographic” naming tradition, with RNA blotting bearing the name Northern blotting and protein transfer to membranes becoming known as Western blotting.作者: 帶來 時間: 2025-3-31 19:33
Western Blotting: Remembrance of Things Past,antigens with hybridomas, and detection of antibody–antigen complexes with radiolabeled staphylococcal protein A and autoradiography. The simplicity and relevance of the method have led to its expansive application as an immunodiagnostic and a ubiquitous research tool in biology and medicine.作者: dragon 時間: 2025-4-1 00:09 作者: Introvert 時間: 2025-4-1 05:31 作者: 消音器 時間: 2025-4-1 09:17 作者: visceral-fat 時間: 2025-4-1 14:07 作者: antidepressant 時間: 2025-4-1 16:27 作者: NICHE 時間: 2025-4-1 19:37 作者: Adjourn 時間: 2025-4-2 00:20
Quantitative Computerized Western Blotting in Detail,e time points are evaluated and the relativities against individual bands compared. This technique can be employed to study humoral responses to complex antigenic mixtures such as allergens and infectious agents, or identify serologic markers for early diagnosis of cancer, autoimmune or infectious diseases, or to monitor patient’s clinical status.作者: FICE 時間: 2025-4-2 05:34